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pET28a MDHM_YEAST
(Plasmid #204231)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204231 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5320
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    MDHM_YEAST
  • Alt name
    Yeast mitochondrial MDH
  • Alt name
    Yeast MDH1, ymMDH
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    993
  • Mutation
    Does not include transit peptide and includes a Met for coding
  • GenBank ID
    NM_001179651.1
  • Entrez Gene
    MDH1 (a.k.a. YKL085W)
  • Promoter Lac
  • Tag / Fusion Protein
    • 6X His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7 Promoter
  • 3′ sequencing primer T7 Terminal
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

UniProt ID: https://www.uniprot.org/uniprotkb/P17505/.

Expression changes based on metabolite starting point. Involvement of MDH in the asp-malate shuttle in yeast extends replicative lifespan and may play a role in calorie-restricted life extension.

For more information, please see the MDH CUREs Community website: https://www.mdhcures.org/.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a MDHM_YEAST was a gift from Malate dehydrogenase CUREs Community (MCC) (Addgene plasmid # 204231 ; http://n2t.net/addgene:204231 ; RRID:Addgene_204231)
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