pET28a hMDH2
(Plasmid #204230)

• Purpose: Bacterial expression of mitochondrial human malate dehydrogenase 2 (hMDH2) transcript variant 1; Use in CURES (integration of research into undergraduate teaching labs).
• Depositing Lab: Malate dehydrogenase CUREs Community (MCC)
• Publication: Expression of His tagged recombinant malate dehydrogenase (MDH) (unpublished)

Backbone

• Vector backbone: pET28a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5232
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: hMDH2
• Alt name: human mitochondrial malate dehydrogenase 2 (hMDH2) transcript variant 1
• Species: H. sapiens (human)
• Insert Size (bp): 978
• Mutation: Does not include transit peptide and includes a Met for coding
• GenBank ID: CR536548.1
• Entrez Gene: MDH2 (a.k.a. DEE51, EIEE51, M-MDH, MDH, MGC:3559, MOR1)
• Promoter: Lac
• Tag / Fusion Protein:
  • 6X His (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7 Promoter
• 3′ sequencing primer: T7 Terminal

Resource Information

• Supplemental Documents:
  • pET28a hMDH2 SnapGene
  • MCC Clone Information Sheet - hMDH2

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For more information, please see the MDH CUREs Community website: https://www.mdhcures.org/