pET28a hMDH1v3 (+TEV)
(Plasmid #204229)

• Purpose: Bacterial expression of human cytosolic malate dehydrogenase isoform 1 transcript variant 3 (hMDH1v3); Use in CURES (integration of research into undergraduate teaching labs).
• Depositing Lab: Malate dehydrogenase CUREs Community (MCC)
• Publication: Expression of His tagged recombinant malate dehydrogenase (MDH) (unpublished)

Backbone

• Vector backbone: pET28a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5206
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: hMDH1v3
• Alt name: human cytosolic MDH
• Species: H. sapiens (human)
• Insert Size (bp): 1107
• Mutation: Inserted a G after ATG to maintain kozak site
• GenBank ID: NM_00119911.1
• Entrez Gene: MDH1 (a.k.a. DEE88, EIEE88, HEL-S-32, KAR, MDH-s, MDHA, MGC:1375, MOR2)
• Promoter: Lac
• Tag / Fusion Protein:
  • 6X His (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7 Promoter
• 3′ sequencing primer: T7 Terminal

Resource Information

• Supplemental Documents:
  • hMDH1V3 (+TEV) SnapGene
  • MCC Clone Information Sheet - hMDH1v3

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

UniProt ID: https://www.uniprot.org/uniprotkb/P40925/entry#P40925-2

Please note: The Uniprot page includes information for all three transcripts. This MDH transcript variant is NOT considered the canonical isoform. See MDH variant alignment map for details.

For more information, please see the MDH CUREs Community website: https://www.mdhcures.org/.