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CMV-Halo-10-PFN1-Y6D
(Plasmid #204223)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204223 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA-Halo expression vector
  • Backbone manufacturer
    Gunther Hollopeter, Cornell University
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PFN1
  • Species
    H. sapiens (human)
  • Mutation
    Y6D
  • Entrez Gene
    PFN1 (a.k.a. ALS18)
  • Promoter CMV
  • Tag / Fusion Protein
    • Halo, TEV site, SGLRSRAQAS linker (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

PFN1(Y6D) was synthesized and inserted into Halo-expression vector by Genscript

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CMV-Halo-10-PFN1-Y6D was a gift from Jessica Henty-Ridilla (Addgene plasmid # 204223 ; http://n2t.net/addgene:204223 ; RRID:Addgene_204223)

  • For your References section:

    Visualizing molecules of functional human profilin. Pimm ML, Liu X, Tuli F, Heritz J, Lojko A, Henty-Ridilla JL. Elife. 2022 Jun 6;11:e76485. doi: 10.7554/eLife.76485. 10.7554/eLife.76485 PubMed 35666129
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