CMV-Halo-10-PFN1
(Plasmid
#204222)
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PurposeExpression of Halo-Profilin-1 in tissue culture cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 204222 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA-Halo expression vector
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Backbone manufacturerGunther Hollopeter, Cornell University
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePFN1
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SpeciesH. sapiens (human)
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Entrez GenePFN1 (a.k.a. ALS18)
- Promoter CMV
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Tag
/ Fusion Protein
- Halo, TEV site, SGLRSRAQAS linker (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
PFN1 was synthesized and inserted into Halo-expression vector by Genscript
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CMV-Halo-10-PFN1 was a gift from Jessica Henty-Ridilla (Addgene plasmid # 204222 ; http://n2t.net/addgene:204222 ; RRID:Addgene_204222) -
For your References section:
Visualizing molecules of functional human profilin. Pimm ML, Liu X, Tuli F, Heritz J, Lojko A, Henty-Ridilla JL. Elife. 2022 Jun 6;11:e76485. doi: 10.7554/eLife.76485. 10.7554/eLife.76485 PubMed 35666129