Skip to main content
Addgene

Dendra2-NS2B
(Plasmid #204218)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204218 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    Dendra2 Tubulin
  • Modifications to backbone
    Removed Tubulin and Inserted NS2B
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Dengue (Dengue Type 2) NS2B
  • Species
    Dengue Virus Serotype , Strain NGC
  • Insert Size (bp)
    399
  • Entrez Gene
    POLY (a.k.a. DENV_gp1, polyprotein gene)
  • Promoter CMV
  • Tag / Fusion Protein
    • Dendra2 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Sac1 (not destroyed)
  • 3′ cloning site BamH1 (unknown if destroyed)
  • 5′ sequencing primer CMV-Forward
  • 3′ sequencing primer SV40-pArev
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pcDNA_DENV2-NS2B3_V5. https://www.addgene.org/115906/

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Dendra2-NS2B was a gift from Partha Mondal (Addgene plasmid # 204218 ; http://n2t.net/addgene:204218 ; RRID:Addgene_204218)

  • For your References section:

    Super-resolution optical microscopy reveals accumulation of photoactivable dengue protein (Dendra2-NS2B) in the endoplasmic reticulum. Aravinth S, Varghese JM, Pant N, Mondal PP. Sci Rep. 2025 Mar 26;15(1):10358. doi: 10.1038/s41598-025-94135-6. 10.1038/s41598-025-94135-6 PubMed 40133359
Related items: