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Addgene

pCDNA3- SREBP-1 (A317-318; L340)
(Plasmid #204195)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204195 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA3.1(+)
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SREBP-1
  • Species
    H. sapiens (human)
  • Mutation
    Gly to Ala 317,318; Gly to Leu 340
  • Entrez Gene
    SREBF1 (a.k.a. HMD, IFAP2, SREBP1, bHLHd1)
  • Tags / Fusion Proteins
    • T7x2 (N terminal on insert)
    • E-tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nhe1 (not destroyed)
  • 3′ cloning site Not1 (not destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Designed with multiple silent mutations to facilitate cloning.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA3- SREBP-1 (A317-318; L340) was a gift from Allan Weissman (Addgene plasmid # 204195 ; http://n2t.net/addgene:204195 ; RRID:Addgene_204195)

  • For your References section:

    SARS-CoV-2 papain-like protease plays multiple roles in regulating cellular proteins in the endoplasmic reticulum. Yang M, Mariano J, Su R, Smith CE, Das S, Gill C, Andresson T, Loncarek J, Tsai YC, Weissman AM. J Biol Chem. 2023 Oct 12;299(12):105346. doi: 10.1016/j.jbc.2023.105346. 10.1016/j.jbc.2023.105346 PubMed 37838170
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