pGP-CMV-jGCaMP8f F366H
(Plasmid #204141)

• Purpose: Fluorescent reporter for calcium imaging in mammalian cells - jGCaMP8f with F366H mutation
• Depositing Lab: Katalin Torok
• Publication: Tran et al Front Cell Neurosci. 2023 May 18;17:1155406. doi: 10.3389/fncel.2023.1155406. eCollection 2023.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: ThermoFisher Invitrogen
• Total vector size (bp): 5224
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: jGCaMP8f F366H
• Species: R. norvegicus (rat), G. gallus (chicken), Synthetic; A. victoria (jellyfish)
• Insert Size (bp): 1269
• Mutation: Phenylalanine 366 changed to histidine
• Promoter: CMV
• Tag / Fusion Protein:
  • N-terminal His tag (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40 pA-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Addgene plasmid 162373 (deposited by GENIE Project)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pGP-CMV-jGCaMP8f was a gift from GENIE Project (Addgene plasmid # 162373 ; http://n2t.net/addgene:162373 ; RRID:Addgene_162373)

We introduced the F366H mutation by site-directed mutagenesis