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Addgene

pGP-CMV-jGCaMP8f L27A
(Plasmid #204140)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204140 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    ThermoFisher Invitrogen
  • Total vector size (bp) 5224
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    jGCaMP8f L27A
  • Species
    R. norvegicus (rat), G. gallus (chicken), Synthetic; A. victoria (jellyfish)
  • Insert Size (bp)
    1269
  • Mutation
    Leucine 27 changed to Alanine
  • Promoter CMV
  • Tag / Fusion Protein
    • N-terminal His tag (N terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Addgene plasmid 162373 (deposited by GENIE Project)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pGP-CMV-jGCaMP8f was a gift from GENIE Project (Addgene plasmid # 162373 ; http://n2t.net/addgene:162373 ; RRID:Addgene_162373)

We introduced the L27A mutation by site-directed mutagenesis

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGP-CMV-jGCaMP8f L27A was a gift from Katalin Torok (Addgene plasmid # 204140 ; http://n2t.net/addgene:204140 ; RRID:Addgene_204140)

  • For your References section:

    Development and characterization of novel jGCaMP8f calcium sensor variants with improved kinetics and fluorescence response range. Tran O, Hughes HJ, Carter T, Torok K. Front Cell Neurosci. 2023 May 18;17:1155406. doi: 10.3389/fncel.2023.1155406. eCollection 2023. 10.3389/fncel.2023.1155406 PubMed 37275778
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