pET30b jGCaMP8f F366H
(Plasmid #204139)

• Purpose: Production of recombinant calcium sensor jGCaMP8f F366H high dynamic range variant in E.coli
• Depositing Lab: Katalin Torok
• Publication: Tran et al Front Cell Neurosci. 2023 May 18;17:1155406. doi: 10.3389/fncel.2023.1155406. eCollection 2023.

Backbone

• Vector backbone: pET30b
• Backbone size w/o insert (bp): 5421
• Total vector size (bp): 6517
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: jGCaMP8f F366H
• Species: R. norvegicus (rat), G. gallus (chicken), Synthetic; A. victoria (jellyfish)
• Insert Size (bp): 1269
• Mutation: Phenylalanine 366 changed to histidine
• Promoter: T7
• Tag / Fusion Protein:
  • N-terminal His tag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 Term

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry