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pET30b jGCaMP8f L27A
(Plasmid #204138)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204138 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET30b
  • Backbone size w/o insert (bp) 5421
  • Total vector size (bp) 6517
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    jGCaMP8f L27A
  • Species
    R. norvegicus (rat), G. gallus (chicken), Synthetic; A. victoria (jellyfish)
  • Insert Size (bp)
    1269
  • Mutation
    Leucine 27 changed to Alanine
  • Promoter T7
  • Tag / Fusion Protein
    • N-terminal His tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET30b jGCaMP8f L27A was a gift from Katalin Torok (Addgene plasmid # 204138 ; http://n2t.net/addgene:204138 ; RRID:Addgene_204138)

  • For your References section:

    Development and characterization of novel jGCaMP8f calcium sensor variants with improved kinetics and fluorescence response range. Tran O, Hughes HJ, Carter T, Torok K. Front Cell Neurosci. 2023 May 18;17:1155406. doi: 10.3389/fncel.2023.1155406. eCollection 2023. 10.3389/fncel.2023.1155406 PubMed 37275778
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