pLenti4/V5-DEST-AERRIE
(Plasmid
#204023)
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PurposeLentiviral vector expressing human long non-coding RNA AERRIE (linc01013)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 204023 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLenti4/V5-DEST™ Gateway™ vector
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Backbone manufacturerThermo Scientific
- Backbone size w/o insert (bp) 6915
- Total vector size (bp) 7944
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Vector typeLentiviral
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namelong non-coding RNA AERRIE
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Alt namelinc01013
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SpeciesH. sapiens (human)
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Insert Size (bp)1029
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GenBank IDNR_038981.1
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Entrez GeneLINC01013 (a.k.a. AERRIE, LINC02921)
- Promoter CMV
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV
- 3′ sequencing primer V5 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti4/V5-DEST-AERRIE was a gift from Reinier Boon (Addgene plasmid # 204023 ; http://n2t.net/addgene:204023 ; RRID:Addgene_204023) -
For your References section:
Long Non-coding RNA Aerrie Controls DNA Damage Repair via YBX1 to Maintain Endothelial Cell Function. Pham TP, Bink DI, Stanicek L, van Bergen A, van Leeuwen E, Tran Y, Matic L, Hedin U, Wittig I, Dimmeler S, Boon RA. Front Cell Dev Biol. 2021 Jan 11;8:619079. doi: 10.3389/fcell.2020.619079. eCollection 2020. 10.3389/fcell.2020.619079 PubMed 33505972