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plko_TRE3G_nlsMyc-Sox9HMGtruncation_PGKpuroR
(Plasmid #203964)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 203964 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLKO
  • Backbone size w/o insert (bp) -2
  • Total vector size (bp) 9428
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SOX9-noHMG
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    678
  • Mutation
    the HMG domain is truncated
  • Entrez Gene
    Sox9 (a.k.a. 2010306G03Rik, mKIAA4243, mSox9)
  • Promoter TRE3G
  • Tag / Fusion Protein
    • myc (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer agctttaggcgtgtacgg
  • 3′ sequencing primer GGCTGCCTTGGAAAAGGCGCAA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    plko_TRE3G_nlsMyc-Sox9HMGtruncation_PGKpuroR was a gift from Elaine Fuchs (Addgene plasmid # 203964 ; http://n2t.net/addgene:203964 ; RRID:Addgene_203964)

  • For your References section:

    The pioneer factor SOX9 competes for epigenetic factors to switch stem cell fates. Yang Y, Gomez N, Infarinato N, Adam RC, Sribour M, Baek I, Laurin M, Fuchs E. Nat Cell Biol. 2023 Jul 24. doi: 10.1038/s41556-023-01184-y. 10.1038/s41556-023-01184-y PubMed 37488435
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