pC1.509
(Plasmid
#203955)
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PurposeLevel 1 self-replicating vector containing a DAPG inducible FnCas12a
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 203955 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonen/a
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePphlF-Cpf1
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SpeciesFrancisella novicida
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
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Addgene Notes
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
11901 toolkit compatible with CyanoGate Kit
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pC1.509 was a gift from Alistair McCormick (Addgene plasmid # 203955 ; http://n2t.net/addgene:203955 ; RRID:Addgene_203955) -
For your References section:
A toolbox to engineer the highly productive cyanobacterium Synechococcus sp. PCC 11901. Victoria AJ, Selao TT, Moreno-Cabezuelo JA, Mills LA, Gale GAR, Lea-Smith DJ, McCormick AJ. Plant Physiol. 2024 May 7:kiae261. doi: 10.1093/plphys/kiae261. 10.1093/plphys/kiae261 PubMed 38713768