pNC863
(Plasmid #20393)

• Depositing Lab: Beverly Errede
• Publication: Hackett et al Yeast. 2006 Apr 15. 23(5):333-49.

Backbone

• Vector backbone: pUC118
• Backbone size w/o insert (bp): 3162
• Vector type: Yeast Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CFP-reporter
• Insert Size (bp): 4487
• Mutation: X=UbiE (see schematic) linker: ek

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SacI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: CTCTCCACTGAGAGAAAA (prim586)

Resource Information

• Supplemental Documents:
  • Schematic from Hackett et al

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See attachments for details

Cassette: CFP-reporter;

UAS: GAL1,10;

X: UbiE;

Linker: ek