pCdOpt-BMX
(Plasmid #203929)

• Purpose: This plasmid contains a codon optimized BleMX drug resistance cassette for generating gene deletions in C. glabrata, C. auris, C. albicans or S. cerevisiae
• Depositing Lab: Scott Briggs
• Publication: Gregor et al mSphere. 2023 Nov 6:e0031123. doi: 10.1128/msphere.00311-23.

Backbone

• Vector backbone: pUCIDT
• Backbone size w/o insert (bp): 2752
• Total vector size (bp): 2752
• Vector type: Yeast Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: BleMX
• Species: Streptoalloteichus hindustanus
• Insert Size (bp): 922
• Mutation: BleMX was codon optimized for Candida albicans or other CTG clade Candida species

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: M13
• 3′ sequencing primer: M13

Resource Information

• Supplemental Documents:
  • 426601502.gb
• A portion of this plasmid was derived from a plasmid made by: Codon Optimized BleMX was gene synthesized by IDT and generated by inserting in pUCIDT vector.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The BleMX is codon optimized for Candida albicans and the TEF1 promoter and TEF1 terminator is used for expression in yeast.