pWR91
(Plasmid #203915)

• Purpose: empty BLINCAR plasmid for payload delivery, no payload, integrates at CRF1 locus
• Depositing Lab: J. Brian Robertson
• Publication: Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi: 10.1128/msphere.00224-23. Epub 2023 Jun 22.

Backbone

• Vector backbone: pWR88
• Backbone manufacturer: Robertson
• Backbone size w/o insert (bp): 8072
• Total vector size (bp): 10118
• Modifications to backbone: A section of S. stipitis genomic DNA around the CRF1 gene was added to pWR88 to serve as a chromosomal integration site.
• Vector type: Yeast Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CRF1 plus flanking
• Species: S. stipitis (yeast)
• Insert Size (bp): 2040
• Entrez Gene: CRF1 (a.k.a. PICST_66716)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SacI (not destroyed)
• 3′ cloning site: SacI (not destroyed)
• 5′ sequencing primer: ATCTTAAGGTGGCCAACTC

Resource Information

• Supplemental Documents:
  • WR91.gb
• A portion of this plasmid was derived from a plasmid made by: coCBG is a codon optimized version of Promega's CBG99 which was synthesized by GenScript

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry