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pLC047 - pL2_p2x35s-CrGLK-tMAS
(Plasmid #203906)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 203906 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSB90
  • Backbone manufacturer
    Mortensen et al., 2019 (doi:10.3389/fpls.2019.00755)
  • Vector type
    Plant Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CrGLK
  • Species
    Catharanthus roseus var. LBE
  • Insert Size (bp)
    2489
  • Mutation
    Silent mutation of BpiI or BsaI cut site for MoClo domestication: T>A 474 bp from start codon
  • Promoter CaMV 2x35S

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (destroyed during cloning)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Contains an additional glycine and serine at the C-terminus as a cloning artifact.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLC047 - pL2_p2x35s-CrGLK-tMAS was a gift from Carolyn Lee-Parsons (Addgene plasmid # 203906 ; http://n2t.net/addgene:203906 ; RRID:Addgene_203906)

  • For your References section:

    The role of the Golden2-like (GLK) transcription factor in regulating terpenoid indole alkaloid biosynthesis in Catharanthus roseus. Cole-Osborn LF, McCallan SA, Prifti O, Abu R, Sjoelund V, Lee-Parsons CWT. Plant Cell Rep. 2024 May 14;43(6):141. doi: 10.1007/s00299-024-03208-9. 10.1007/s00299-024-03208-9 PubMed 38743349
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