pLC025 - pL0_StopCodon (CT)
(Plasmid
#203905)
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PurposeMoClo compatible L0 plasmid containing a stop codon. To allow for proper fusion with CDS1ns L0 plasmids, this plasmid adds an additional glycine and serine to the C-terminal end of a CDS and then adds a stop codon to terminate translation.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 203905 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepAGM1301
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Backbone manufacturerWeber et al., 2011 (DOI:10.1371/journal.pone.0016765)
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameStop codon
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SpeciesSynthetic
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Insert Size (bp)7
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (destroyed during cloning)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLC025 - pL0_StopCodon (CT) was a gift from Carolyn Lee-Parsons (Addgene plasmid # 203905 ; http://n2t.net/addgene:203905 ; RRID:Addgene_203905) -
For your References section:
The role of the Golden2-like (GLK) transcription factor in regulating terpenoid indole alkaloid biosynthesis in Catharanthus roseus. Cole-Osborn LF, McCallan SA, Prifti O, Abu R, Sjoelund V, Lee-Parsons CWT. Plant Cell Rep. 2024 May 14;43(6):141. doi: 10.1007/s00299-024-03208-9. 10.1007/s00299-024-03208-9 PubMed 38743349
