pUN1309 - pL2_pNOS-RLUC-tNOS_tOCS-FLUC-pD4H
(Plasmid
#203901)
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PurposeReporter plasmid for the D4H promoter from C. roseus with firefly luciferase readout and Renilla luciferase normalization in a plant binary vector for Agrobacterium-mediated transformation.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 203901 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSB90
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Backbone manufacturerMortensen et al., 2019 (doi:10.3389/fpls.2019.00755)
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Vector typePlant Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepD4H-FLUC-tOCS; pNOS-RLUC-tNOS
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SpeciesSynthetic; Catharanthus roseus var. LBE
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Insert Size (bp)5278
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (destroyed during cloning)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUN1309 - pL2_pNOS-RLUC-tNOS_tOCS-FLUC-pD4H was a gift from Carolyn Lee-Parsons (Addgene plasmid # 203901 ; http://n2t.net/addgene:203901 ; RRID:Addgene_203901)