pUN381 - pTRV2-CHLH
(Plasmid
#203886)
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PurposeViral silencing plasmid targeting the CHLH gene from C. roseus. For use as a positive control for VIGS experiments.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 203886 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTRV2-GATEWAY
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Backbone manufacturerLiu et al., 2002 (doi: 10.1046/j.1365-313x.2002.01394.x)
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Vector typePlant Expression, RNAi
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCHLH fragment
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SpeciesCatharanthus roseus var. LBE
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Insert Size (bp)499
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUN381 - pTRV2-CHLH was a gift from Carolyn Lee-Parsons (Addgene plasmid # 203886 ; http://n2t.net/addgene:203886 ; RRID:Addgene_203886) -
For your References section:
The role of the Golden2-like (GLK) transcription factor in regulating terpenoid indole alkaloid biosynthesis in Catharanthus roseus. Cole-Osborn LF, McCallan SA, Prifti O, Abu R, Sjoelund V, Lee-Parsons CWT. Plant Cell Rep. 2024 May 14;43(6):141. doi: 10.1007/s00299-024-03208-9. 10.1007/s00299-024-03208-9 PubMed 38743349