pHflu3
(Plasmid
#203881)
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PurposepHI1 with insertion of kanamycin-resistance gene
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 203881 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHI1
- Backbone size w/o insert (bp) 4747
- Total vector size (bp) 6124
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Vector typeBacterial Expression, Yeast Expression, Synthetic Biology
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Selectable markersHIS3
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Kanamycin, 25 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameaminoglycoside O-phosphotransferase APH(3')-IIa
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Alt namekanR
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Alt namenptII
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SpeciesE. coli
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Insert Size (bp)1377
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GenBank IDWP_000572405
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHflu3 was a gift from Bogumil Karas (Addgene plasmid # 203881 ; http://n2t.net/addgene:203881 ; RRID:Addgene_203881)