pHflu2
(Plasmid #203880)

• Purpose: pHI1 with insertion of ampicillin-resistance gene
• Depositing Lab: Bogumil Karas
• Publication: Medium-copy shuttle vectors for E. coli, H. influenzae, and S. cerevisiae with visual and antibiotic markers (unpublished)

Backbone

• Vector backbone: pHI1
• Backbone size w/o insert (bp): 4743
• Total vector size (bp): 5859
• Vector type: Bacterial Expression, Yeast Expression, Synthetic Biology
• Selectable markers: HIS3

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: broad-spectrum class A beta-lactamase TEM-116
• Alt name: ampR
• Alt name: blaTEM
• Species: E. coli
• Insert Size (bp): 1116
• GenBank ID: WP_000027050

Resource Information

• Supplemental Documents:
  • pHflu2.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry