pHflu1
(Plasmid
#203878)
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Purpose(Empty Backbone) pSU20 with addition of CEN6-ARSH4-HIS3 cassette for S. cerevisiae and RK2 origin of conjugal transfer
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 203878 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSU20
- Backbone size (bp) 2341
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Vector typeBacterial Expression, Yeast Expression, Synthetic Biology
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Selectable markersHIS3
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHflu1 was a gift from Bogumil Karas (Addgene plasmid # 203878 ; http://n2t.net/addgene:203878 ; RRID:Addgene_203878)