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Addgene

Tet-O-FUW-NR4A3-P2A-mCherry
(Plasmid #203863)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 203863 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    Tet-O-FUW
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NR4A3
  • Species
    H. sapiens (human)
  • Entrez Gene
    NR4A3 (a.k.a. CHN, CSMF, MINOR, NOR1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Depositor confirms S240G mutation in NR4A3 does not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Tet-O-FUW-NR4A3-P2A-mCherry was a gift from Andrea Califano (Addgene plasmid # 203863 ; http://n2t.net/addgene:203863 ; RRID:Addgene_203863)
  • For your References section:

    Systematic elucidation and pharmacological targeting of tumor-infiltrating regulatory T cell master regulators. Obradovic A, Ager C, Turunen M, Nirschl T, Khosravi-Maharlooei M, Iuga A, Jackson CM, Yegnasubramanian S, Tomassoni L, Fernandez EC, McCann P, Rogava M, DeMarzo AM, Kochel CM, Allaf M, Bivalacqua T, Lim M, Realubit R, Karan C, Drake CG, Califano A. Cancer Cell. 2023 May 8;41(5):933-949.e11. doi: 10.1016/j.ccell.2023.04.003. Epub 2023 Apr 27. 10.1016/j.ccell.2023.04.003 PubMed 37116491