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Addgene

AAV-U6-sgRNA-hSyn1-DIO-EYFP-KASH
(Plasmid #203849)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 203849 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    AAV
  • Vector type
    AAV, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EYFP-KASH
  • Species
    M. musculus (mouse)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV-U6-sgRNA-hSyn1-DIO-EYFP-KASH was a gift from Ivo Spiegel (Addgene plasmid # 203849)

  • For your References section:

    Meso-seq for in-depth transcriptomics in ultra-low amounts of FACS-purified neuronal nuclei. Apelblat D, Roethler O, Bitan L, Keren-Shaul H, Spiegel I. Cell Rep Methods. 2022 Jul 25;2(8):100259. doi: 10.1016/j.crmeth.2022.100259. eCollection 2022 Aug 22. 10.1016/j.crmeth.2022.100259 PubMed 36046622
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