pAAV-CaMKII-jGCaMP7f-WPRE
(Plasmid
#203848)
-
PurposeExpression of GCaMP7f in CaMKII expressing cells
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 203848 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backboneAAV
-
Vector typeAAV
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature30°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namejGCaMP7f
-
SpeciesSynthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pAAV-CaMKII-jGCaMP7f-WPRE was a gift from Ivo Spiegel (Addgene plasmid # 203848 ; http://n2t.net/addgene:203848 ; RRID:Addgene_203848) -
For your References section:
NDNF interneurons in layer 1 gain-modulate whole cortical columns according to an animal's behavioral state. Cohen-Kashi Malina K, Tsivourakis E, Kushinsky D, Apelblat D, Shtiglitz S, Zohar E, Sokoletsky M, Tasaka GI, Mizrahi A, Lampl I, Spiegel I. Neuron. 2021 Jul 7;109(13):2150-2164.e5. doi: 10.1016/j.neuron.2021.05.001. Epub 2021 May 25. 10.1016/j.neuron.2021.05.001 PubMed 34038743