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Addgene

pAAV-Ef1a-fDIO-rc[Chronos-eGFP]-WPRE
(Plasmid #203847)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 203847 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    AAV
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Chronos
  • Species
    Synthetic
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-Ef1a-fDIO-rc[Chronos-eGFP]-WPRE was a gift from Ivo Spiegel (Addgene plasmid # 203847 ; http://n2t.net/addgene:203847 ; RRID:Addgene_203847)

  • For your References section:

    NDNF interneurons in layer 1 gain-modulate whole cortical columns according to an animal's behavioral state. Cohen-Kashi Malina K, Tsivourakis E, Kushinsky D, Apelblat D, Shtiglitz S, Zohar E, Sokoletsky M, Tasaka GI, Mizrahi A, Lampl I, Spiegel I. Neuron. 2021 Jul 7;109(13):2150-2164.e5. doi: 10.1016/j.neuron.2021.05.001. Epub 2021 May 25. 10.1016/j.neuron.2021.05.001 PubMed 34038743
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