pAAV-Ef1a-fDIO-mRuby3-2A-dCre
(Plasmid
#203845)
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PurposeFlp-dependent enzymatically inactive Cre and mRuby3 expression
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 203845 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneAAV
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namedCre
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SpeciesM. musculus (mouse)
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MutationEnzymatically inactive Cre
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Tag
/ Fusion Protein
- mRuby3-2A (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-Ef1a-fDIO-mRuby3-2A-dCre was a gift from Ivo Spiegel (Addgene plasmid # 203845 ; http://n2t.net/addgene:203845 ; RRID:Addgene_203845) -
For your References section:
Single genomic enhancers drive experience-dependent GABAergic plasticity to maintain sensory processing in the adult cortex. Roethler O, Zohar E, Cohen-Kashi Malina K, Bitan L, Gabel HW, Spiegel I. Neuron. 2023 Jun 20:S0896-6273(23)00399-9. doi: 10.1016/j.neuron.2023.05.026. 10.1016/j.neuron.2023.05.026 PubMed 37354902