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Addgene

pAAV-hSyn-Kir2.1Mut-2A-mScarlet-KASH
(Plasmid #203841)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 203841 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    AAV
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Kir2.1
  • Species
    M. musculus (mouse)
  • Mutation
    G144A, Y145A, G146A
  • Entrez Gene
    Kcnj2 (a.k.a. IRK1, Kcnf1, Kir2.1)
  • Tag / Fusion Protein
    • 2A-mScarlet-KASH (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-hSyn-Kir2.1Mut-2A-mScarlet-KASH was a gift from Ivo Spiegel (Addgene plasmid # 203841 ; http://n2t.net/addgene:203841 ; RRID:Addgene_203841)

  • For your References section:

    Single genomic enhancers drive experience-dependent GABAergic plasticity to maintain sensory processing in the adult cortex. Roethler O, Zohar E, Cohen-Kashi Malina K, Bitan L, Gabel HW, Spiegel I. Neuron. 2023 Jun 20:S0896-6273(23)00399-9. doi: 10.1016/j.neuron.2023.05.026. 10.1016/j.neuron.2023.05.026 PubMed 37354902
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