pGL4.11_Nued2_E1_dAP1-2
(Plasmid
#203837)
-
PurposeLuciferase reporter plasmid for Igf1 enhancer 1 with both AP-1 sites knocked out
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 203837 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepGL4.11_Nued2
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namedAP1-2
-
SpeciesM. musculus (mouse)
-
MutationKO of both AP1 sites
Cloning Information
- Cloning method Gibson Cloning
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pGL4.11_Nued2_E1_dAP1-2 was a gift from Ivo Spiegel (Addgene plasmid # 203837 ; http://n2t.net/addgene:203837 ; RRID:Addgene_203837) -
For your References section:
Single genomic enhancers drive experience-dependent GABAergic plasticity to maintain sensory processing in the adult cortex. Roethler O, Zohar E, Cohen-Kashi Malina K, Bitan L, Gabel HW, Spiegel I. Neuron. 2023 Jun 20:S0896-6273(23)00399-9. doi: 10.1016/j.neuron.2023.05.026. 10.1016/j.neuron.2023.05.026 PubMed 37354902