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Addgene

pCgVchcrRNA
(Plasmid #203815)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 203815 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJYS1Ptac
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    CRISPR(BsaI)
  • Species
    Vibrio cholera

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid can also be transformed in Corynebacterium glutamicum, grown at 30 C

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCgVchcrRNA was a gift from Sheng Yang (Addgene plasmid # 203815 ; http://n2t.net/addgene:203815 ; RRID:Addgene_203815)

  • For your References section:

    RNA-Guided DNA Transposition in Corynebacterium glutamicum and Bacillus subtilis. Yang S, Zhu J, Zhou X, Zhang J, Li Q, Bian F, Zhu J, Yan T, Wang X, Zhang Y, Yang J, Jiang Y, Yang S. ACS Synth Biol. 2023 Jul 21;12(7):2198-2202. doi: 10.1021/acssynbio.3c00193. Epub 2023 Jun 22. 10.1021/acssynbio.3c00193 PubMed 37348112
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