pBsVchDonor
(Plasmid
#203814)
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PurposeDNA donor for transposition
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 203814 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepKS1
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Vector typeBacterial Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Erythromycin, 150 μg/mL μg/mL
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Growth Temperature30°C
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Growth Strain(s)Top10
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Growth instructionsAllow to grow for 2 or more days
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameMini-Tn, aprE cassette
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SpeciesVibrio cholera
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Plasmid can also be transformed in Bacillus subtilis, grown at 37 C
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBsVchDonor was a gift from Sheng Yang (Addgene plasmid # 203814 ; http://n2t.net/addgene:203814 ; RRID:Addgene_203814) -
For your References section:
RNA-Guided DNA Transposition in Corynebacterium glutamicum and Bacillus subtilis. Yang S, Zhu J, Zhou X, Zhang J, Li Q, Bian F, Zhu J, Yan T, Wang X, Zhang Y, Yang J, Jiang Y, Yang S. ACS Synth Biol. 2023 Jul 21;12(7):2198-2202. doi: 10.1021/acssynbio.3c00193. Epub 2023 Jun 22. 10.1021/acssynbio.3c00193 PubMed 37348112