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Addgene

pBsVchCAST-pyrE
(Plasmid #203813)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 203813 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHT43
  • Vector type
    Bacterial Expression, CRISPR
  • Selectable markers
    Chloramphenicol

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC, CRISPR(pyrE)
  • Species
    Vibrio cholera

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid can also be transformed in Bacillus subtilis, grown at 37 C

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBsVchCAST-pyrE was a gift from Sheng Yang (Addgene plasmid # 203813 ; http://n2t.net/addgene:203813 ; RRID:Addgene_203813)

  • For your References section:

    RNA-Guided DNA Transposition in Corynebacterium glutamicum and Bacillus subtilis. Yang S, Zhu J, Zhou X, Zhang J, Li Q, Bian F, Zhu J, Yan T, Wang X, Zhang Y, Yang J, Jiang Y, Yang S. ACS Synth Biol. 2023 Jul 21;12(7):2198-2202. doi: 10.1021/acssynbio.3c00193. Epub 2023 Jun 22. 10.1021/acssynbio.3c00193 PubMed 37348112
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