pAcCas12nHS_RV6_PT1_empty
(Plasmid
#203810)
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PurposePlasmid containing a CMV-driven NLS-AcCas12n-PT1-NLS(human-codonoptimized)cassette and a U6-driven sgRNA_V6 cassette(containing BsaI sites for spacer insertion). Carbenicillin resistance.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 203810 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonePuc
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAcCas12nHS_RV6_PT1
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Alt nameCas12U4
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SpeciesSynthetic
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAcCas12nHS_RV6_PT1_empty was a gift from Quanjiang Ji (Addgene plasmid # 203810 ; http://n2t.net/addgene:203810 ; RRID:Addgene_203810) -
For your References section:
Cas12n nucleases, early evolutionary intermediates of type V CRISPR, comprise a distinct family of miniature genome editors. Chen W, Ma J, Wu Z, Wang Z, Zhang H, Fu W, Pan D, Shi J, Ji Q. Mol Cell. 2023 Jun 27:S1097-2765(23)00463-X. doi: 10.1016/j.molcel.2023.06.014. 10.1016/j.molcel.2023.06.014 PubMed 37402371
