pCMV-miR21
(Plasmid #20381)

• Depositing Lab: Bryan Cullen
• Publication: Zeng et al RNA. 2003 Jan . 9(1):112-23.

Backbone

• Vector backbone: pCMV-PL
• Backbone size w/o insert (bp): 3200
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: miR21
• Species: H. sapiens (human)
• Insert Size (bp): 93
• Entrez Gene: MIR21 (a.k.a. MIRN21, hsa-mir-21, miR-21, miRNA21)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV promoter

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry