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Addgene

pAcCas12nHS_ABE_D3_empty
(Plasmid #203809)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 203809 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Puc
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ABE_D3
  • Species
    Synthetic
  • Promoter CMV

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAcCas12nHS_ABE_D3_empty was a gift from Quanjiang Ji (Addgene plasmid # 203809 ; http://n2t.net/addgene:203809 ; RRID:Addgene_203809)

  • For your References section:

    Cas12n nucleases, early evolutionary intermediates of type V CRISPR, comprise a distinct family of miniature genome editors. Chen W, Ma J, Wu Z, Wang Z, Zhang H, Fu W, Pan D, Shi J, Ji Q. Mol Cell. 2023 Jun 27:S1097-2765(23)00463-X. doi: 10.1016/j.molcel.2023.06.014. 10.1016/j.molcel.2023.06.014 PubMed 37402371
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