pLV-SFFV-Llgl1-WPRE-UbC-Emerald
(Plasmid
#203805)
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PurposeLentiviral vector plasmid expressing mouse Llgl1 under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 203805 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHRsinUbEm
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameLlgl1
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Alt nameLLGL scribble cell polarity complex component 1
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Alt nameLgl1
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SpeciesM. musculus (mouse)
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Insert Size (bp)3111
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Entrez GeneLlgl1 (a.k.a. Lgl1, Llglh, Mgl1)
Cloning Information for Gene/Insert 1
- Cloning method Gibson Cloning
Gene/Insert 2
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Gene/Insert nameEmerald
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Alt nameEmGFP
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SpeciesA. victoria
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Insert Size (bp)717
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLV-SFFV-Llgl1-WPRE-UbC-Emerald was a gift from Richard Eva & Bart Nieuwenhuis (Addgene plasmid # 203805 ; http://n2t.net/addgene:203805 ; RRID:Addgene_203805)