pLV-SFFV-RAB10-WPRE-UbC-Emerald
(Plasmid #203804)

• Purpose: Lentiviral vector plasmid expressing human RAB10 under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
• Depositing Labs: Richard Eva, Bart Nieuwenhuis
• Publication: Plasmid DNA for mammalian expression and viral vectors (unpublished)

Backbone

• Vector backbone: pHRsinUbEm
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: RAB10
• Alt name: RAB10, member RAS oncogene family
• Alt name: Ras-Related GTP-Binding Protein
• Species: H. sapiens (human)
• Insert Size (bp): 603
• Entrez Gene: RAB10

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning

Gene/Insert 2

• Gene/Insert name: Emerald
• Alt name: EmGFP
• Species: A. victoria
• Insert Size (bp): 717

Resource Information

• Supplemental Documents:
  • pLV-SFFV-RAB10-WPRE-UbC-Emerald.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry