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Addgene

AAV-sCAG-eGFP-ACAP1 F280E
(Plasmid #203801)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 203801 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    N/A
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ACAP1
  • Alt name
    ArfGAP with coiled-coil, ankyrin repeat and PH domains 1
  • Alt name
    CENTB1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2958
  • Mutation
    F280E
  • Entrez Gene
    ACAP1 (a.k.a. CENTB1)
  • Tag / Fusion Protein
    • eGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site AgeI (not destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV-sCAG-eGFP-ACAP1 F280E was a gift from Richard Eva & Bart Nieuwenhuis (Addgene plasmid # 203801 ; http://n2t.net/addgene:203801 ; RRID:Addgene_203801)
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