Luc-SIRT1 mutant 3'UTR
(Plasmid #20380)

• Depositing Lab: Charles Lowenstein
• Publication: Yamakuchi et al Proc Natl Acad Sci U S A. 2008 Sep 9. 105(36):13421-6.

Backbone

• Vector backbone: pMIR-REPORT
• Backbone size w/o insert (bp): 6470
• Vector type: Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: SIRT1
• Alt name: sirtuin (silent mating type information regulation 2 homolog) 1
• Species: H. sapiens (human)
• Insert Size (bp): 1420
• Mutation: mutant 3'UTR of SIRT1 (ACACCCACCAAGGACCATTAGTCCGAGA)
• Entrez Gene: SIRT1 (a.k.a. SIR2, SIR2L1, SIR2alpha)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PmeI (not destroyed)
• 3′ cloning site: SacI (not destroyed)
• 5′ sequencing primer: N/A

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There are mismatches between Addgene's sequence and NCBI sequence for SIRT1 UTR. Deposit is aware of these changes and doesn't think they affect function.