AAV-sCAG-eGFP-RAB11
(Plasmid
#203799)
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PurposeAAV vector plasmid expressing human RAB11 fused to eGFP under a short variant of the CAG (sCAG) promoter
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 203799 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneN/A
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRAB11
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Alt nameRAB11A
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Alt nameRAB11A, Member RAS Oncogene Family
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SpeciesH. sapiens (human)
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Insert Size (bp)1407
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Entrez GeneRAB11A (a.k.a. YL8)
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Tag
/ Fusion Protein
- eGFP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site AgeI (not destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AAV-sCAG-eGFP-RAB11 was a gift from Richard Eva & Bart Nieuwenhuis (Addgene plasmid # 203799 ; http://n2t.net/addgene:203799 ; RRID:Addgene_203799)