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pLV-SFFV-PIK3CA H1047R-WPRE-UbC-Emerald
(Plasmid #203798)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 203798 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHRsinUbEm
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    PIK3CA
  • Alt name
    Phosphatidylinositol-4,5-Bisphosphate 3-Kinase Catalytic Subunit Alpha
  • Alt name
    P110alpha
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3207
  • Mutation
    H1047R
  • Entrez Gene
    PIK3CA (a.k.a. CCM4, CLAPO, CLOVE, CWS5, HMH, MCAP, MCM, MCMTC, PI3K, PI3K-alpha, p110-alpha)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NotI (not destroyed)

Gene/Insert 2

  • Gene/Insert name
    Emerald
  • Alt name
    EmGFP
  • Species
    A. victoria
  • Insert Size (bp)
    717

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV-SFFV-PIK3CA H1047R-WPRE-UbC-Emerald was a gift from Richard Eva & Bart Nieuwenhuis (Addgene plasmid # 203798 ; http://n2t.net/addgene:203798 ; RRID:Addgene_203798)

  • For your References section:

    PI 3-kinase delta enhances axonal PIP3 to support axon regeneration in the adult CNS. Nieuwenhuis B, Barber AC, Evans RS, Pearson CS, Fuchs J, MacQueen AR, van Erp S, Haenzi B, Hulshof LA, Osborne A, Conceicao R, Khatib TZ, Deshpande SS, Cave J, Ffrench-Constant C, Smith PD, Okkenhaug K, Eickholt BJ, Martin KR, Fawcett JW, Eva R. EMBO Mol Med. 2020 Aug 7;12(8):e11674. doi: 10.15252/emmm.201911674. Epub 2020 Jun 17. 10.15252/emmm.201911674 PubMed 32558386
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