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pminiTol2-4kb wnt3 promoter-Fzd1mApple
(Plasmid #203795)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 203795 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pminiTol2
  • Backbone size w/o insert (bp) 3430
  • Vector type
    Zebrafish Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Fzd1
  • Alt name
    frizzled class receptor 1
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    6684
  • Entrez Gene
    fzd1 (a.k.a. fz10, fz3, zg03, zg10, zgc:194761, zgc:194774)
  • Promoter 4kb Wnt3 promoter
  • Tag / Fusion Protein
    • mApple (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pminiTol2-4kb wnt3 promoter-Fzd1mApple was a gift from Thorsten Wohland (Addgene plasmid # 203795 ; http://n2t.net/addgene:203795 ; RRID:Addgene_203795)

  • For your References section:

    Wnt3 distribution in the zebrafish brain is determined by expression, diffusion and multiple molecular interactions. Veerapathiran S, Teh C, Zhu S, Kartigayen I, Korzh V, Matsudaira PT, Wohland T. Elife. 2020 Nov 25;9:e59489. doi: 10.7554/eLife.59489. 10.7554/eLife.59489 PubMed 33236989
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