pminiTol2-4kb wnt3 promoter-PMTmApple
(Plasmid
#203794)
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PurposeTol2 based genomic integration of zebrafish 4kb Wnt3 promoter driving the expression of PMTmApple for zebrafish transgenesis
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 203794 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepminiTol2
- Backbone size w/o insert (bp) 3320
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Vector typeZebrafish Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePMT
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Alt nameplasma membrane targeted mApple
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)4761
- Promoter 4kb Wnt3 promoter
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Tag
/ Fusion Protein
- mApple (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pminiTol2-4kb wnt3 promoter-PMTmApple was a gift from Thorsten Wohland (Addgene plasmid # 203794 ; http://n2t.net/addgene:203794 ; RRID:Addgene_203794) -
For your References section:
Wnt3 distribution in the zebrafish brain is determined by expression, diffusion and multiple molecular interactions. Veerapathiran S, Teh C, Zhu S, Kartigayen I, Korzh V, Matsudaira PT, Wohland T. Elife. 2020 Nov 25;9:e59489. doi: 10.7554/eLife.59489. 10.7554/eLife.59489 PubMed 33236989