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pminiTol2-4kb wnt3 promoter-Wnt3EGFP
(Plasmid #203793)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 203793 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pminiTol2
  • Backbone size w/o insert (bp) 3565
  • Vector type
    Zebrafish Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Wnt3
  • Alt name
    Wingless-type MMTV integration site family, member 3
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    5829
  • Entrez Gene
    wnt3
  • Promoter 4kb Wnt3 promoter
  • Tag / Fusion Protein
    • mEGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pminiTol2-4kb wnt3 promoter-Wnt3EGFP was a gift from Thorsten Wohland (Addgene plasmid # 203793 ; http://n2t.net/addgene:203793 ; RRID:Addgene_203793)

  • For your References section:

    Modulating the expression level of secreted Wnt3 influences cerebellum development in zebrafish transgenics. Teh C, Sun G, Shen H, Korzh V, Wohland T. Development. 2015 Nov 1;142(21):3721-33. doi: 10.1242/dev.127589. Epub 2015 Sep 22. 10.1242/dev.127589 PubMed 26395493
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