EGFR∆550-580-mEGFP
(Plasmid
#203788)
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PurposeNumber and brightness analysis of EGFR oligomerization
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 203788 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRK5
- Backbone size w/o insert (bp) 2847
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameEGFR
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SpeciesH. sapiens (human)
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Insert Size (bp)4374
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Mutationaa 550-580 deleted
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Entrez GeneEGFR (a.k.a. ERBB, ERBB1, ERRP, HER1, NISBD2, NNCIS, PIG61, mENA)
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Tag
/ Fusion Protein
- mEGFP (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EGFR∆550-580-mEGFP was a gift from Thorsten Wohland (Addgene plasmid # 203788 ; http://n2t.net/addgene:203788 ; RRID:Addgene_203788) -
For your References section:
The dependence of EGFR oligomerization on environment and structure: A camera-based N&B study. Balasubramanian H, Sankaran J, Pandey S, Goh CJH, Wohland T. Biophys J. 2022 Dec 6;121(23):4452-4466. doi: 10.1016/j.bpj.2022.11.003. Epub 2022 Nov 5. 10.1016/j.bpj.2022.11.003 PubMed 36335429