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Addgene

EGFR∆550-580-mEGFP
(Plasmid #203788)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 203788 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRK5
  • Backbone size w/o insert (bp) 2847
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    EGFR
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4374
  • Mutation
    aa 550-580 deleted
  • Entrez Gene
    EGFR (a.k.a. ERBB, ERBB1, ERRP, HER1, NISBD2, NNCIS, PIG61, mENA)
  • Tag / Fusion Protein
    • mEGFP (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EGFR∆550-580-mEGFP was a gift from Thorsten Wohland (Addgene plasmid # 203788 ; http://n2t.net/addgene:203788 ; RRID:Addgene_203788)

  • For your References section:

    The dependence of EGFR oligomerization on environment and structure: A camera-based N&B study. Balasubramanian H, Sankaran J, Pandey S, Goh CJH, Wohland T. Biophys J. 2022 Dec 6;121(23):4452-4466. doi: 10.1016/j.bpj.2022.11.003. Epub 2022 Nov 5. 10.1016/j.bpj.2022.11.003 PubMed 36335429
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