PMT-mScarlet_2
(Plasmid
#203780)
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PurposeBrightness calibration
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 203780 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRK5
- Backbone size w/o insert (bp) 2949
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePMT-mScarlet dimer
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Alt nameplasma membrane targeted mScarlet dimer
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SpeciesSynthetic
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Insert Size (bp)1488
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PMT-mScarlet_2 was a gift from Thorsten Wohland (Addgene plasmid # 203780 ; http://n2t.net/addgene:203780 ; RRID:Addgene_203780) -
For your References section:
The dependence of EGFR oligomerization on environment and structure: A camera-based N&B study. Balasubramanian H, Sankaran J, Pandey S, Goh CJH, Wohland T. Biophys J. 2022 Dec 6;121(23):4452-4466. doi: 10.1016/j.bpj.2022.11.003. Epub 2022 Nov 5. 10.1016/j.bpj.2022.11.003 PubMed 36335429