PMT-mApple
(Plasmid
#203771)
-
PurposeBrightness calibration
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 203771 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepRK5
- Backbone size w/o insert (bp) 2949
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert namePMT-mApple
-
Alt nameplasma membrane targeted mApple
-
SpeciesSynthetic
-
Insert Size (bp)783
Cloning Information
- Cloning method Gibson Cloning
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
PMT-mApple was a gift from Thorsten Wohland (Addgene plasmid # 203771 ; http://n2t.net/addgene:203771 ; RRID:Addgene_203771) -
For your References section:
Simultaneous spatiotemporal super-resolution and multi-parametric fluorescence microscopy. Sankaran J, Balasubramanian H, Tang WH, Ng XW, Rollin A, Wohland T. Nat Commun. 2021 Mar 19;12(1):1748. doi: 10.1038/s41467-021-22002-9. 10.1038/s41467-021-22002-9 PubMed 33741958