GST TopBP1 (aa 32-1522) His
(Plasmid #20375)

• Depositing Lab: Aziz Sancar
• Publication: Choi et al Nucleic Acids Res. 2009 Jan 12. ():.

Backbone

• Vector backbone: pGEX6P1
• Backbone manufacturer: Amersham
• Backbone size w/o insert (bp): 4900
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: TopBP1
• Alt name: TOPBP1
• Alt name: topoisomerase II beta binding protein 1
• Species: H. sapiens (human)
• Insert Size (bp): 4566
• Mutation: Starts at aa32
• Entrez Gene: TOPBP1 (a.k.a. Dpb11, TOP2BP1)
• Tag / Fusion Protein:
  • 6xHis (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: pGEX 5'
• 3′ sequencing primer: pGEX 3'

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

TopBP1 cDNA KIAA00259 was cloned by Weei-Chin Lin in EcoRI site of pGEX6P1. I added a His6 tag at the 3' end using the oligos TopBPF3 and TopBPR4 for PCR, then digested the PCR product with XhoI and replaced the XhoI fragment from pGEX6P1TopBP1 with the PCR product to generate pGEX6P1TopBP1His.

NOTE: This plasmid was originally called full-length, and it is considered to have full length functionality in its original publication from the Sancar lab.